The discovery of biologically active molecules and isolation to crystallize into promising extracts put specific requirement on bioassays to be utilized for purpose.

Bioassays are simple, rapid, reproducible and cost effective in comparison with other assays. Bioassays are prerequisite to burgeon in High Throughput Screening. Thus this leads us to bioactivity guided fractionation and isolated domain of potential drug leads with help of Reverse Pharmacology, System Biology, and Evidence Based Medicine.

In this context, our R&D team has developed certain bioassays and these are ready for offering.

• Quantity of sample required:
  1 gm sample in a properly closed air tight container. In case the sample is scarce and expensive, lesser quantities can also suffice (Please specify the assays for us to provide the minimum sample quantity requirement).
• Turn Around Time:
  The table indicates typical turn around time required for performing the assays. Please enquire about the exact turn around time, at the time of order
• Terms and conditions:
  Samples with poor solubility profile in aqueous buffers may not be suitable for analysis.
• IC50 / EC50 values can be calculated only for active samples. For samples which do not have sufficient activity the results may not be amenable to statistical analysis or graph plotting and they will be reported as such. In most cases, IC50 will be calculated using linear regression analysis.
• Details about methodology adopted, importance and relevance of these assays are available on request. For a few assays, positive control data may not be available.
• For all bio-chemical assays, each sample is normally tested at five different concentrations, in duplicate. However for some specific assays a different approach is adopted to obtain reliable results.
• The cost for cell based assays includes determination of the measured parameter at nine pre- determined concentrations, for herbal extracts (0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, 100 μg/ml) and for pure compounds (10-9, 10-8, 10-7, 10-6, 10-5 M), with 4 replicates per concentration. Parallel cell viability estimation is also included in the same cost. These concentrations can be modified as per user request.
• For Ames test, the test substance will be screened at 6 non-cytotoxic concentrations, in triplicate, in the presence and absence of metabolic activator.
• Samples should be preferably free of preservatives for testing in cellular assays. In case preservatives have been added, information should be provided as such samples will require additional testing of preservative controls for obtaining reliable results.
• The results reported by the lab will be applicable to the given test conditions of Indus Extracts assay protocol. It should be noted sometimes results can vary across labs due to variation in assay conditions.
• In rare occasions, some assays may be temporarily unavailable due to reagent unavailability. Further new assays are being added regularly, please request for the available list of assays at the time of ordering.

Disclaimer: The in vitro bioassays given in this list / catalogue are being offered under company’s contract research program. In-house standardized assay protocols will be used to perform these assays. The company is committed to ensure that utmost care is taken to adhere to specified protocols towards providing accurate and factual results to the users of this service. However, further use of the results and analytical reports provided by Indus Extracts for any commercial purposes is the sole responsibility of the user and Indus Extracts does not make any representation that the use of the bioassay reports will not infringe any intellectual property rights of third parties. In no event shall Indus Extracts be liable to any party for any direct, indirect, special or other consequential damages for any commercial use of the bioassay reports provided by Indus Extracts.